DNA microarray technologies for measuring protein–DNA interactions

DNA microarray technologies for measuring protein-DNA interactions.

DNA-binding proteins have key roles in many cellular processes, including transcriptional regulation and replication. Microarray-based technologies permit the high-throughput identification of binding sites and enable the functional roles of these binding proteins to be elucidated. In particular, microarray readout either of chromatin immunoprecipitated DNA-bound proteins (ChIP-chip) or of DNA ...

Technologies for Measuring Particulates

Microarray-based genomic DNA profiling technologies in clinical molecular diagnostics.

BACKGROUND Microarray-based genomic DNA profiling (MGDP) technologies are rapidly moving from translational research to clinical diagnostics and have revolutionized medical practices. Such technologies have shown great advantages in detecting genomic imbalances associated with genomic disorders and single-gene diseases. CONTENT We discuss the development and applications of the major array pl...

Physicochemical perspectives on DNA microarray and biosensor technologies.

Detection and sequence-identification of nucleic acid molecules is often performed by binding, or hybridization, of specimen "target" strands to immobilized, complementary "probe" strands. A familiar example is provided by DNA microarrays used to carry out thousands of solid-phase hybridization reactions simultaneously to determine gene expression patterns or to identify genotypes. The underlyi...

Extrapolating Traditional DNA Microarray Statistics to the Tiling and Protein Microarray Technologies

A credit to the microarray technology is its broad application. Two experiments the tiling microarray experiment, and the protein microarray experiment are exemplars of the microarray's versatility. With the technology's expanding list of uses, the corresponding bioinformatics must evolve in step. There currently exists a rich literature developing statistical techniques for analyzing tradition...